Figure 3.

Effects of variation in ATPS1 on sulfate assimilation. Col-0, atps1, Bay-0, Shahdara, 004B, and 004S plants were grown for 2 weeks on Murashige and Skoog-agarose vertical plates in a controlled-environment room. A and B, APR activity (A) and glutathione (GSH) content (B) were measured in shoots. C and D, The plants were incubated for 4 h with 0.2 mm [³⁵S]sulfate, and the incorporation of ³⁵S into glutathione, Cys, and proteins (C) and glucosinolates (GLS; D) was quantified. Results are presented as means ± sd from three pools of four plants. Different letters mark significantly different values at P < 0.05. FW, Fresh weight.