Figure 2.

Induced changes in the Tre6P content of Arabidopsis rosettes at night. Ethanol-inducible TPS plants (TPS29.2 and TPS31.3) were grown in soil with a 12-h photoperiod. Wild-type plants (Col-0) and plants expressing the AlcR ethanol-binding transcription factor (AlcR) were grown as controls. Four-week-old plants were sprayed with water (white bars) or 2% (v/v) ethanol (black bars) at the beginning of the night. Pools of 10 rosettes were harvested 12 h later at the EN for the determination of Tre6P (A), trehalose (B), Suc (C), Glc (D), Fru (E), starch (F), maltose (G), and maltotriose (H). Data are means ± sd (n = 3). Significant difference between ethanol- and water-treated plants from the same genotype are indicated by asterisks (Student’s t test): *P < 0.05, **P < 0.01, and ***P < 0.001. FW, Fresh weight.