Figure 3.

Changes in subanther locations of At5g62080-GFP and At5g07230-GFP during anther development in Arabidopsis. CLSM of the fluorescence of anthers at progressive developmental stages in plants transformed with the respective genes including the promoter and coding regions attached to GFP was performed. GFP fluorescence is shown in green, and chlorophyll autofluorescence is shown in magenta. The same settings (laser power and detection gain) were used for direct comparison of the developing anthers. At stage 10, the tapetum began undergoing programmed cell death. L, Locule; M, microspore; T, tapetum; Td, tetrad. Scale bars are in μm.