Figure 5.

Nontargeting of structurally modified At5g62080-GFP and GFP-tagged native non-type III LTPs to exine of stage 10 microspores of transformed Arabidopsis plants. A, Schematic diagram of the disulfide bonds between the four pairs of Cys residues in At5g62080. B, Absence of At5g62080-GFP with its first (C1-MU-GFP) or eighth (C8-MU-GFP) Cys residue modified to Ala on microspores. Nonmodified LTP-GFP was used as a positive control. GFP fluorescence is shown in green, and exine autofluorescence is shown in magenta. Scale bars are in μm. C, Absence of type IX LTP-GFP (from At3g07450 and At3g52130; expressed mostly in inflorescences in Affymetrix microarray data) and type I LTP-GFP (from At3g51600; expressed abundantly in leaves) on microspores. In transformed plants, the three LTP-GFPs were driven by the At5g62080 promoter. Scale bars are in μm.