Figure 9.

Transcriptome comparison between wild-type (WT) and la1-ref mutant nodes using RNA-SEQ. A and B, Functional categories of the DEGs in la1-ref mutants using MapMan: up-regulated genes (A) and down-regulated genes (B). C and D, qRT-PCR validation of the selected DEGs: up-regulated genes (C) and down-regulated genes (D). The relative expression levels of DEGs were examined by fragments per kilobase of exon per million fragments mapped in RNA-SEQ. Three corresponding biological replicate samples were performed for the wild type and la1-ref in qRT-PCR, and three technical validations were performed in each biological replication. ZmActin1 (GRMZM2G126010) was used as an internal reference. Error bars indicate the sd between biological replicates.