Figure 6.

Time-dependent ABA-GE uptake of membrane vesicles from yeast expressing AtABCC1 and AtABCC2 in the absence (A) or presence (B) of 4 mm MgATP. Membrane vesicles were obtained from pYES3-AtABCC2 (circles), pNEV-AtABCC1 (squares), or the empty vector pNEV (EV; triangles) transformed yeast strain YMM36, which is deleted in the yeast ABCC genes Ycf1, Ybt1, and Bpt1. ABA-GE uptake was determined at an ABA-GE concentration of 40 nm. Each data point represents the mean ± sd of three experimental replicates from one representative experiment out of three experiments with independent vesicle preparations.