Figure 2.

Root length of serk mutants in response to BL. A, Root length of wild-type seedlings (ecotype Columbia [Col-0]) and serk1-3 and serk3-2 single and double mutants at 0 nm BL (top), 1 nm BL (middle), and 10 nm BL (bottom). All assays were performed in the presence of 1 µm BRZ. B, Root growth assays of the serk1-3 and serk3-2 single and double mutants compared with the wild type at different BL concentrations. Root lengths were measured at 8 DAG and plotted relative to the BRZ-treated control. The serk1-3 single mutant does not have a clear BL-dependent phenotype, whereas the serk3-2 mutant never reaches the wild-type root length and does not show any inhibition in response to BL. The serk1-3serk3-2 double mutant is almost completely unresponsive toward BL. C, Root lengths relative to the BRZ-treated control at 4 DAG. For all measurements, n ≥ 15 roots, and error bars indicate se. [See online article for color version of this figure.]