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. 2013 Oct 30;8(10):e77433. doi: 10.1371/journal.pone.0077433

Figure 5. Change in the expression of epithelial and mesenchymal cell markers after GSI IX treatment in human cholangiocarcinoma.

Figure 5

(A) SZ1 and (B) TFK1 cells were treated with control (DMSO) and GSI (5 µM, 20 µM and 40 µM) for 48 h and 96 h. The expression of EMT markers: E-cadherin, N-cadherin, Snail, Beta Catenin and Vimentin were analyzed by Western blot. β-actin was used as a loading control. Both A) SZ1 and (B) TFK1 cells showed an increase of epithelial marker E-cadherin, Beta Catenin and an decrease of Snail and Vimentin. However, N-cadherin expression was unchanged for both cholangiocarcinoma cell lines.