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A) Envelope length in amino acids and N-linked glycosylation sites (NxT/S). B) V1 to V5 loop and signal peptide (SP) lengths in the amino acid sequence sets analysed. Red cluster nucleotide sequences, blue Spanish nucleotide sequences, black ancient nucleotide sequences and green subtype B nucleotide sequences. P values for comparison between 2 groups shown with horizontal black bars were calculated using a 2-tailed Mann-Whitney test.
