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Number of litters and pups (WT and P0) in each group and each study arm are demonstrated. Sildenafil citrate (0.4 mg/ml in drinking water) was administered from embryonic day (E) 12.5 and all measurements were taken at E 18.5. For ultrasound biomicroscopy, due to necessity to ID pups accurately immediately after scanning (as WT and P0 exist within the same litter), not all pups underwent ultrasound biomicroscopy (numbers in parentheses are those that did.).
