Figure 2. Fetal and placental weights following Sildenafil citrate treatment.

Fetal weights (A,B), placental weights (C) and fetal:placental weight ratios (D) at embryonic day 18.5 in wild-type (WT) and placental-specific Igf2 knockout (P0) mice. Pregnant dams were allowed access to water with (N = 19 litters) or without (N = 23 litters) Sildenafil citrate (SC, 0.4 mg/ml). In figures 2A,C and D, Mean+SEM is shown. Figure 2B demonstrates fetal weights as frequency distribution curves for individual pups; WT control (black solid curve), WT SC (black dashed curve), P0 control (grey solid curve) and P0 SC (grey dashed curve). Vertical dashed line denotes the 5^th centile of WT control fetal weights (1.03 g). Individual pup n’s: WT control n = 95, WT SC n = 82, P0 control n = 88, P0 SC n = 77. Statistical analyses of genotype and treatment were performed by Generalized Linear Mixed Models test followed by Sequential Sidak multiple comparisons test. ***P<0.001 WT v P0, ^≠ P<0.05 P0 water v P0 SC group.