Figure 1.
Normalized methylation-sensitive high-resolution melting (MS-HRM) standard curves of MLH1 and MGMT in CRC. (A) Profile of fluorescence obtained at the melting temperature for serial dilutions of methylated DNA (100-0%) and the melting plot for the MGMT gene. (B) Profile of fluorescence obtained at the melting temperature for serial dilutions of methylated DNA (100-0%) and the melting plot for the MLH1 gene. MLH1, DNA mismatch repair gene mutator L homoloue 1; MGMT, DNA repair gene O6-methylguanine-DNA methyltransferase; CRC, colorectal cancer.