Table III.
MS-HRM assay of peripheral blood and colorectal tissue samples of CRC patients using various cut-off values.
| MGMT | MLH1 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
|
|
|
||||||||
| Cut-off (%) | Leukocytes, n (%) | Normal tissue, n (%) | Tumor tissue, n (%) | P-value* | P-value* | Leukocytes, n (%) | Normal tissue, n (%) | Tumor tissue, n (%) | P-value* |
| 0 | 7 (15.9) | 9 (20.5) | 13 (29.5) | 0.282 | - | 12 (27.3) | 14 (31.8) | 13 (29.5) | 0.897 |
| 1 | 7 (15.9) | 7 (15.9) | 12 (27.3) | 0.302 | - | 12 (29.5) | 12 (29.5) | 11 (25.0) | 0.962 |
| 5 | 2 (4.5) | 3 (6.8) | 10 (22.7) | 0.014 | 0.645a, 0.035b, 0.013c | 5 (11.4) | 11 (25.0) | 9 (20.5) | 0.251 |
*
χ2 test; leukocytes vs. normal tissue;
b
tumor tissue vs. normal tissue; and
c
tumor tissue vs. leukocytes.
MS-HRM, methylation-sensitive high-resolution melting; CRC, colorectal cancer; MGMT, DNA repair gene O6-methylguanine-DNA methyltranserferase; MLH1, DNA mismatch repair gene mutator L homologue 1.