Figure 2.

Effect of oridonin on apoptosis in MHCC97-H cells. The cells were treated with the indicated concentrations of oridonin for 24 h. Apoptosis was determined using Annexin V/PI staining. Data are presented as the mean ± SD of three independent experiments. Z represents cells that were treated with oridonin (100 μM) and the caspase-9 inhibitor Z-LEHD-FMK (20 μM).^*P<0.05 compared with the control cells (LSD t-test). ^ΔP<0.01 compared with the cells that were treated with 100 μM oridonin alone (t-test). LSD, least significant difference; PI, propidium iodide.