Figure 2.

LB-mediated actin disruption induces apoptosis via enhancement of Bax expression in PTEN-null PC3M prostate cancer cell lines. (A) Disruption of actin microfilament and induction of apoptosis. The morphology, immunostained F-actin (rhodamine-phalloidine) and DNA (DAPI) of PC3M cells, with or without treatment (150 nM LB for 24 h), were examined by phase contrast microscopy and confocal microscopy. (B and C) Cleaved PARP, as well as Bcl-2 and Bax proteins, were expressed following LB treatment. PC3M cells were treated with either different concentrations (0, 50, 150 and 300 nM) of LB for 24 h (B) or with different times (0, 6, 12 and 24 h) under fixed 150 nM LB (C). Total cell extracts (30 μg) were used for western blotting with anti-PARP, -Bcl-2 and -Bax antibodies. LB, latrunculin B; PTEN, phosphatase and tensin homolog; PARP, poly (ADP-ribose) polymerase.