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. 2013 Aug 26;6(5):1383–1389. doi: 10.3892/ol.2013.1547

Figure 3.

Figure 3

LB induces DSBs via the MEK/Erk pathway in PTEN-null prostate cancer cells. (A and B) Levels of γH2AX protein expressed following LB treatment. PC3M cells were treated with either LB (0, 50, 150 and 300 nM) for 24 h (A) or with 150 nM LB for 0, 3, 12 and 24 h (B). (C) Levels of pErk protein induced after LB treatment. PC3M cells were treated with LB (0, 50, 150 and 300 nM) for 24h. (D) Effect of LB treatment, with or without U0126, on γH2AX expression. PC3M cells were treated with LB (150 nM) in the presence or absence of U0126 (20 μM) for 24 h. Nuclear extracts (5 μg) for γH2AX and total cell extracts (30 μg) for pErk were used for western blotting with anti-γH2AX and anti-pErk antibodies, respectively. LB, latrunculin B; DSBs, double-strand breaks; MEK, mitogen-activated protein kinase kinase; Erk, extracellular signal-regulated kinase; PTEN, phosphatase and tensin homolog; pErk, phosphorylated Erk.