Figure 4.

U0126 attenuates LB-mediated apoptotic events in PTEN-null PC3M cells. (A) Levels of PARP cleavage and pAkt after treatment with LB in the presence or absence of U0126 and LY294002. PC3M cells were treated with LB (150 nM) in the presence or absence of U0126 (20 μM) and LY294002 (10 μM), respectively, for 24 h. (B and C) Reduction of LB-mediated morphological change and PARP cleavage by Erk inhibition, but not by ROS inhibition. PC3M cells were treated with LB (150 nM) in the presence or absence of U0126 (20 μM) and NAC (10 mM), respectively, for 24 h. Total cell extracts (30 μg) were used for western blotting with anti-PARP and anti-pAkt antibodies, respectively. LB, latrunculin B; PTEN, phosphatase and tensin homolog; PARP, poly (ADP-ribose) polymerase; pAKT, phosphorylated protein kinase B; Erk, extracellular signal-regulated kinase; ROS, reactive oxygen species; NAC, N-acetyl-L-cysteine.