Fig. 2.

Expression of vimentin and desmin proteins in transdifferentiating rat HSCs. (A) HSCs were collected after the indicated number of days in culture and ten µg of RIPA-soluble cell lysates were separated by SDS-PAGE. Protein expression was assessed by Western blotting. Approximate molecular weights are indicated on the left of the panels. (B) The same experiments shown in panel A were done at least three times using other lots of HSCs. The intensities of chemiluminescent signals are indicated by means±standard deviations. The signal intensities on day seven were set to one for each protein analyzed. Asterisks indicate significant (P<0.05) differences compared to proteins on day seven by ANOVA.