Figure 7.

Heat-elicited intronless isoforms of known heat-responsive alternatively spliced genes. (A) Hsrω: gene model showing intronless nuclear transcript subset RB:RF and spliced cytoplasmic subset RD:RA:RE (top); average normalized log² exon expression for the RB:RF subset and constitutive exons (left); and real-time PCR data confirming early RB:RF expression relative to RpL11 and delayed expression of the RD:RA:RE subset (right). The PCR data were log² transformed for visual clarity and nontransformed data with error bars (±SE of the mean) are shown in Figure S6. (B) Dnaj-1: gene model showing intronless transcript RB and spliced isoform RA (top); average normalized log² exon expression showing strong induction of the RB transcript relative to both transcripts during heat stress (bottom). (C) CG6000/CR449007: gene model showing intronless RC transcript and spliced RB/CR44907 isoform and proximity to Hsp68 (top); average normalized log² exon expression showing strong induction of both transcripts.