Figure 8.

Elucidating expression complexity of alternatively transcribed genes upregulated during thermal stress in the exon data. (A) stv: gene model showing the long and short transcript subsets derived by alternative transcription and alternative splicing (top); average normalized log² exon expression for the RA:RE:RF, RB:RC:RG subsets and RD and constitutive exons (middle); and real-time PCR data relative to RpL11 showing no expression variation of the RA:RE:RF subset, accumulation of the primary transcript during heat stress, and induction of the RB:RC:RG subset and RD isoform by alternative transcription only during recovery. (B) Hsc70-4: gene model showing isoforms derived by alternative transcription (top); average normalized log² exon expression of the RA, and RD isoforms, and RB:RF subset (middle); and real-time PCR data showing only accumulation of the primary transcript during heat stress (bottom). (C) Hsc70-3: gene model showing isoforms derived by alternative transcription (top); average normalized log² exon expression of long (RB, recovery induced), and short (RC, stress induced) (middle); and real time PCR data for only the primary transcript showing accumulation during heat stress (bottom). The PCR data were log² transformed for visual clarity and nontransformed data with error bars (±SE of the mean) are shown in Table S7.