Figure 7.

Rescue of the β-spectrin overexpression phenotype in the wing by coexpression of α-spectrin. Doubly heterozygous males carrying autosomal UAS-α-Spec³⁷ and UAS-β-Spec⁹⁵ transgenes were crossed to homozygous MS1096-Gal4 females to drive their expression during wing development. (A–C) Three classes of wing phenotypes were distinguished in the adult progeny. (D) Western blot analysis with anti-myc tag antibody detected the expected progeny classes expressing transgene alone (lanes 2 and 3), both transgenes (lane 1), or neither transgene (lane 4). When reared at 25°, adults expressing UAS-β-Spec⁹⁵ under control of MS1096-Gal4 produced a severe wing phenotype (B). Flies expressing neither transgene (A) or expressing just UAS-α-Spec³⁷ were indistinguishable with no detectable wing phenotype (no transgene shown). A third class of flies was observed in which wings had a mildly cupped phenotype, corresponding to the class overexpressing both α- and β-spectrin (C).