Figure 2.

The role of pp-1 in hyphal network formation. (A) PP1-GFP shows nuclear localization during chemotropic growth in germlings. (B) The closed-circuit experimental design used for microarray analysis. The 4- to 6-hr subpopulation loop is marked in the dashed box. (C) Phenotypic analyses of conidial germination and hyphal network formation in wild type corresponding to identical time points as the 8-hr microarray time course. Arrows denote germling hyphal fusion events. Bar, 10 μm. (D) Visual representation of clustered gene expression profiles from Hierarchical Clustering Explorer analysis (de Hoon et al. 2004). Across each horizontal row (representing a single gene from the 3- to 8-hr germination time course), red indicates the highest dependence on PP1 for expression and green indicates the lowest dependence.