Skip to main content
. 2013 Nov;195(3):899–913. doi: 10.1534/genetics.113.154393

Figure 4.

Figure 4

Ephrin signaling has asymmetric requirements in amphid commissure ventral guidance. (A) Amphid outgrowth defects display left-hand bias in Eph signaling mutants; representative example from efn-1(e96). Bar, 10 μm. (B) Quantitation of axon guidance defects in left vs. right hand AWB, ASI, or AFD axons in efn-1 or vab-1(e118) mutants. unc-40 and sax-3 do not show detectable left–right bias in penetrance. Green bars are right-side axons and red bars, left. (C) Transformation of ASEL into an ASER-like fate by lsy-6 does not alter the left-hand bias in guidance defects in vab-1(e2027) background. (D) Asymmetry in Pefn-2-mCherry (juEx2737) expression as determined by 4D lineage analysis at 335 min postfertilization; anterior is to the left and left is up. Blue nuclei are bilateral pairs that show symmetrical expression of efn-2, green nuclei are members of bilateral pairs for which only the right-hand side expresses efn-2, red nuclei are members of pairs that express only on the left-hand side. Gray nuclei are nonexpressing (grayscale depth code). See Table S2 for list of expressing nuclei. (E) Loss of efn-2 function increases asymmetry of efn-1 mutants, does not affect vab-1(e2027) or vab-1(dx31) null mutants, and decreases penetrance and asymmetry in vab-1(kd).