Figure 1.

RMSD (left) and mean bias (right) for method-of-moments estimates of F_ST under different sequencing coverage (2×, 6×, and 20×). We compared the accuracy of the new method, which does not rely on genotype calling $\left({\widehat{F}}_{\text{ST.Ev}}\right)$, and a method based on allele frequencies estimated from called genotypes $\left({\widehat{F}}_{\text{ST.GC}}\right)$ (see Materials and Methods). We simulated 20 individuals for each population and 10,000 sites for each scenario.