Skip to main content
. 2013 Oct 14;5(10):1767–1779. doi: 10.3390/toxins5101767

Figure 2.

Figure 2

(a) Affinity chromatography of raw protein extract on acid-treated Sepharose 6B. (b) Chromatography of affinity-bound protein on Superdex 75. (c) SDS-PAGE of proteins purified from S. ebulus blossoms. The amounts of protein per well were ebulin f (11.5 µg), SELfd (11.1 µg), ebulin blo (9.5 µg) and SELblo (10.8 µg). The markers were, from top to bottom: SNAI (Mr 136 kDa), BSA (Mr 68 kDa), Ng b (Mr 58 kDa), ovalbumin (Mr 45 kDa), SNAIV (Mr 30 kDa) and trypsin inhibitor (Mr 20 kDa). 2-ME, 2-mercaptoethanol.