Figure 2.

4-MU treatment leads to increased apoptosis in SEB-exposed leukocytes in vitro. Spleen cells from C57BL/6 mice were treated with 4-MU (0.1, and 0.5 mM) or vehicle control (DMSO) and then stimulated with SEB (2 μg/mL). The effect of 4-MU on SEB-induced apoptosis was determined 48 h later by Annexin V/PI (A) and TUNEL (B) assays, respectively. The level of apoptosis in individual immune cell subsets was determined by staining the spleen cells with phenotype-specific fluorescently-labeled mAbs followed by TUNEL staining (C).