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. 2013 Oct 17;5(10):1814–1826. doi: 10.3390/toxins5101814

Figure 3.

Figure 3

4-MU treatment suppresses SEB-induced hyaluronic acid synthase expression and accumulation of soluble HA in the lungs. The effect of 4-MU on soluble HA levels in the lungs of SEB-exposed mice was determined by treating the mice with 4-MU (450 mg/mouse i.p.) or vehicle control (5% gum arabic) one day prior and on the day of SEB exposure (20 µg/50 μL PBS). The levels of lung hyaluronic acid and mRNA levels of HAS were determined 24 h later. HAS mRNA levels in whole lung extracts were determined by real-time RT-PCR (A). Hyaluronic acid levels in bronchoalveolar lavage fluid (BALF) were determined by ELISA (B). Asterisks indicate statistically significant difference when compared with the levels from vehicle-exposed mice, p ≤ 0.05. Number sign indicates statistically significant difference when compared to PBS exposed mice, p ≤ 0.05.