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Analysis of fusion protein binding to HLA-DR1-bearing cells. Binding of radiolabelled SEB to DAP.3-DR1 was carried out in competition with unlabelled SEB, SEB-MBP, nΔ81SEB-MBP, cΔ19SEB-MBP, and cΔ34SEB-MBP. The insert shows a representative Scatchard analysis for binding of SEB to DAP.3-DR1 cells. The inserts represent plots of bound/free (ordinate) vs. bound (abscissa).
