Figure 5.

CD8⁺ T cells express chemokines upon activation, but not MCP-1 or MIP-2. RNase protection assays were performed on RNA extracted from CD8⁺ T cells after 4-hour stimulation with PDB and ionomycin or plate-bound anti-CD3 at varying concentrations. With the exception of RANTES (and possibly IP-10), there was no constitutive chemokine expression by T cells without stimulation. MIP-1α, MIP-1β, and Ltn are most prominently expressed by the T cells upon stimulation, as well as faint levels of IP-10, TCA-3, and RANTES message. No MIP-2 or MCP-1 expression was evident, even with significant overexposure (not shown).