Figure 9.

TNF-R1 (p55) is required for in vivo expression of MCP-1 triggered by CD8⁺ T cell recognition. RNase protection assays were performed on RNA extracted from whole-lung homogenates after adoptive transfer of 5 × 10⁶ CD8⁺ T cell clone 40-2. SPC-HA–transgenic animals were bred into the TNF-R1–deficient (p55^–/–) background and compared with wild-type HA⁺ littermates. Lungs were harvested 24 hours after adoptive transfer and assayed for chemokine expression. MCP-1 expression was absent in the HA⁺ p55^–/– recipients but present in the HA⁺ p55^+/+ animals. IP-10 expression was similar in both.