Skip to main content
. 2000 Sep 15;106(6):793–802. doi: 10.1172/JCI10052

Figure 5.

Figure 5

Reduction of CCR2 expression by oxidized linoleic acid. THP-1 cells were incubated for 24 hours with the indicated concentrations of native linoleic acid (open circles), oxidized linoleic acid (filled circles), 9-HODE (filled triangles), and 13-HODE (filled diamonds). Due to cytotoxic effects observed at high concentrations, the final concentration of oxidized linoleic acid was limited to 10 μg/mL. CCR2 protein was estimated by flow cytometry using phycoerythrin-conjugated anti–CCR2 IgG. Nonspecific fluorescence, obtained by labeling of the cells with phycoerythrin-conjugated human isotype IgG, was subtracted, and the median CCR2-specific fluorescence is shown as percentage of protein expression. The values represent the means ± SD of three independent experiments. Inset: The cells were incubated for 24 hours with the indicated concentrations of native (open circles) or oxidized (filled circles) linoleic acid. CCR2 mRNA was estimated by semiquantitative RT-PCR and normalized to GAPDH mRNA estimated under identical conditions.