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. Author manuscript; available in PMC: 2013 Oct 31.
Published in final edited form as: J Immunol. 2010 Jun 4;185(1):10.4049/jimmunol.0903767. doi: 10.4049/jimmunol.0903767

Figure 3. IFN-γ and perforin are required for Vγ4-mediated anti-tumor immune responses.

Figure 3

CD44high Vγ4 γδ T cells were sorted from either B6 wild-type mice, or perforin−/− or IFNγ−/− mice spleens and lymph nodes, and stimulated with UC3 antobody in the presence of IL-2 for 8 days. B16 F0 tumor cells (2×105 cells/mouse) were mixed with these expanded Vγ4 γδ T cells (0.5×105 cells/mouse), injected into B6 TCR δ−/− mice (n =10 per each group) subcutaneously. Tumor growth was observed and recorded daily, Tumor size greater than 4×4 mm2 was considered positive. Data represent 3 independent experiments. *, p < 0.05. **, p < 0.01.