Figure 7. Effects of GAB2 on HIF-1α transcriptional activity and protein expression.

(a) Melanocytes expressing NRAS^G12D and GAB2^WT+NRAS^G12D or MM415 melanoma cells with stable knockdown of GAB2 (Sh1 and Sh2) were treated with DMSO or CoCl₂ (100 µM) for 24 hours. Total RNA from these cells was analyzed for mRNA expression by qRT-PCR. qRT-PCR experiments were performed in triplicate. Error bars indicate ± standard error **p<0.01.

(b) Cell lysates from these cells were examined for HIF-1α protein expression by western blotting. Tubulin was used as loading control.