. Author manuscript; available in PMC: 2014 Sep 12.

Published in final edited form as: Cell. 2013 Sep 12;154(6):10.1016/j.cell.2013.08.035. doi: 10.1016/j.cell.2013.08.035

Table 1.

Summary of isotopic labeling patterns and solid state NMR measurements on Aβ40 fibrils derived from patient I.

sample	uniformly ¹⁵N,¹³C-labeled residues	selectively ¹³C-labeled sites	NMR measurements¹
A	residues 1-40		2D fpRFDR, NCAX, NCOCX, RAD, PAR, and TEDOR; 3D NCACX and NCOCX; ¹⁵N- and ¹³C-BARE
B	F19, V24, G25, A30, I31, L34, M35		2D fpRFDR, RAD, NCACX
C	F20, D23, V24, K28, G29, A30, I31		2D fpRFDR, RAD, NCACX; fsREDOR
D	H14, Q15, A21, V36, G37		2D fpRFDR, RAD, NCACX; ¹⁵N- and ¹³C-BARE
E	K16, F19, A21, E22, I32, V36		2D fpRFDR, RAD, NCACX; ¹⁵N- and ¹³C-BARE
F		A2 and A21 methyl; V12, F20, I31, G38 carbonyl	PITHIRDS-CT
G		A30 methyl, V18 carbonyl	PITHIRDS-CT

Definitions of acronyms: fpRFDR = finite-pulse radio-frequency-driven recoupling; NCACX = ¹⁵N-¹³C_α-¹³C_x chemical shift correlation; NCOCX = ¹⁵N-¹³CO-¹³C_x chemical shift correlation; RAD = radio-frequency-assisted diffusion; PAR = proton-assisted recoupling; TEDOR = transferred echo double resonance; BARE = backbone recoupling; fsREDOR = frequency-selective rotational echo double resonance; PITHIRDS-CT = constant-time π-pulse recoupling