Fig. 4.

Secretion of HSA by bgs and wild type strains. Strains, either untransformed or carrying expression plasmids for HSA, were induced on methanol, and the volumes of extracellular medium, standardized to equal cell numbers, were probed with anti-HSA antibody. Spot western images captured with a ChemiImager 5500 were quantified with integrated densitometry values (IDV) analysis. Negative refers to the untransformed parent strain. WT refers to the parental wild type strain containing the HSA expression plasmid