Table 1. Cloned bgs mutants.

Bioinformatic analysis suggested that the predicted proteins have similarity to characterized proteins with the indicated structural or functional properties. β;galactosidase activity was measured in the extracellular fractions of cells induced on methanol growth medium. Residual β-galactosidase activity of the wild type parent strain containing the lacZ expression plasmid was subtracted, and the adjusted values are reported as units/ L OD600. Doubling times were calculated on YPD medium. The values are averages of three trials

Mutant	Gene locus disrupted	Comments/putative function	β-gal secretion	Doubling time (min)
bgs1	PAS_chr1-1_0268	Subunit of dynactin	8.7 ± 0.4	130
bgs2	PAS_chr3_1240	SKIP_SNW family	0.7 ± 0.2	240
bgs3	PAS_chr2-1_0743	Unknown function	2.9 ± 0.1	110
bgs4	PAS_chr3_1240	Same as bgs2	–	–
bgs5	PAS_chr2-1_0744	Heavy chain dynein	13 ± 0.7	120
bgs6	PAS_chr2-2_0224	TRAPP (transport protein particle)	4.2 ± 0.1	140
bgs7	PAS_chr1-4_0031	Pleckstrin-like, nuclear transport	39 ± 5.6	110
bgs8	PAS_chr4_0054	Nuclear transport protein	1.5 ± 0.2	140
bgs9	PAS_chr1-4_0456	Polyphosphatidy-linositol phosphatase	1.0 ± 0.1	120
bgs10	PAS_chr3_0754	Aminoacyl tRNA synthetase	0.6 ± 0.1	140
bgs11	PAS_chr1-1_0068	Staphylococcal nuclease-like (SNase-like)	4.6 ± 0.2	110
bgs12	PAS_chr1-4_0443	Cytoplasmic dynein, intermediate chain	8.6 ± 0.6	110
bgs13	PAS_chr2-1_0124	Protein kinase C	13 ± 0.5	160
bgs14	PAS_chr2-1_0124	Same as bgs13	–	–
bgs15	PAS_chr2-1_0124	Same as bgs13	–	–