Table 2.

Expression of different reporter proteins secreted by bgs and wild type strains

Reporter protein	Wild type	bgs2	bgs3	bgs7	bgs13
HRP	100	71	360	84	99
Lipase	100	85	96	112	137
SLPI	100	93	103	218	214

The values are from enzyme activity and spot western data and represent averages of at least three trials of the strains containing each reporter plasmid. Wild type activity was defined as 100 % for HRP (59 U/L OD₆₀₀) and lipase (3.5 U/L OD₆₀₀). For SLPI expression, supernatant volumes corresponding to equivalent numbers of cells were spotted onto nitrocellulose and probed with anti-myc antibodies. Spot western images captured with a ChemiImager 5500 were subjected to Integrated Densitometry Values (IDV) analysis. 100 % wild type SLPI secreted corresponds to ~2 mg/l