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. Author manuscript; available in PMC: 2013 Oct 31.
Published in final edited form as: Mol Genet Genomics. 2009 Feb 17;281(5):10.1007/s00438-009-0428-8. doi: 10.1007/s00438-009-0428-8

Figure 1. Generation of the gene-activation virus.

Figure 1

A – Schematic of the elements in the gene activation virus. The gene-activation cassette includes a polyadenylation signal from bovine growth hormone (pA), Gal4 responsive promoter (UAS) and a synthetic element with a splice donor (SD). Additional elements are the viral long terminal repeats (LTR), Ψ+ viral packaging signals and LacZ. B – integration of the virus in the intron of a gene. In the presence of Gal4-VP16 transcription is initiated from the UAS and splicing should occur from the splice donor to the splice acceptor (SA) of the following exon. The resulting RNA will have a portion of the gene-activation cassette on the 5′ end. C – Infection of Pac2 zebrafish cells with virus from two clones, 10D11 and 9D4 determined using QPCR. Negative ΔΔCt values indicate more proviral inserts per cell compared to the GT186 virus. D - Infection of zebrafish embryos with virus from two clones, 10D11 and 9D4. Number of proviral inserts determined using QPCR.