FIGURE 6:

Myosin Va depletion induces perinuclear clustering of endocytic recycling pathway membrane compartments. HeLa cells transfected with control siRNA or myosin Va siRNA for 72 h were fixed, permeabilized, and colabeled with antibodies to the TfR and TGN46 (A), EEA1 and GM130 (B), or LBPA and TGN46 (C). (D) Quantification of the fluorescence intensity of TfR, EEA1, or LBPA in the pericentrosomal region relative to the total cellular fluorescence intensity and expressed as a “compaction factor.” Data were normalized to the control (mean ± SEM; n = 30–50 cells). *p <10⁻⁴, Student's t test.