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. 2004 Mar 4;23(6):1348–1359. doi: 10.1038/sj.emboj.7600143

Figure 4.

Figure 4

Genetic interactions between Rsc4 bromodomains and HATs. (A) rsc4 BDTs− mutants are lethal in combination with gcn5Δ. Key indicates genotype of strains. YBC1622 was transformed with rsc4 BDTs− plasmids or controls: pRS314.RSC4 (p1060), pRS314, pRS314.rsc4-2 (p1083), pRS314.rsc4-7 (p1086), or pRS314.rsc4-11 (p1089). Strains were grown at 28°C on selective media with or without 5-FOA to enforce the loss of pRS316.RSC4. (B) rsc4 BDTs− mutants are viable in combination with esa1-L327S at 28°C. YBC1796 was transformed with the rsc4 BDTs− plasmids listed above and plated as above. (C) rsc4-2 is lethal in combination with esa1-L327S only at elevated temperatures. YBC627 and YBC1796 were transformed with pRS314.RSC4 or pRS314.rsc4-2, and plated to 5-FOA to enforce loss of pRS316.RSC4. The 10-fold dilutions were spotted to YPD and grown for 2–3 days at the indicated temperatures.