FIGURE 8:

Displacement of Sec16 by removal of COPII coat proteins from tER sites. (A) Drug-induced displacement of Sec23 from tER sites to ribosomes. The procedure was the same as in Figure 7A, except that Sec23 was tagged with FRB-GFP. (B) Drug-induced displacement of Sec31 from tER sites. Gene replacement was used to tag Sec23 and Shl23 with FRB and to tag Sec31 with GFP. The Sec31-GFP pattern was then visualized in the absence of rapamycin (–Rap) or after incubation for 10 min in the presence of 1 μg/ml rapamycin (+Rap). Fluorescence and differential interference contrast images were combined, with the same exposure times for both panels. (C) Drug-induced displacement of Sec16 from tER sites. The procedure was the same as in B, except that Sec16 was tagged with GFP. Scale bars, 5 μm.