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. 2013 Nov 1;24(21):3350–3357. doi: 10.1091/mbc.E13-05-0257

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© 2013 Ivanova et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Cdc10 is released from chromatin upon DNA damage. (A) Loading of Cdc10 on cdc22 and cdc18 promoters was measured by ChIP analysis of chromatin extracts isolated from untreated or treated (0.1% MMS, 1 h at 30ºC) cultures of WT, ∆cds1, ∆chk1, ∆cds1∆chk1, or ∆rad3 cells. Endogenous Cdc10 is HA tagged, and the levels of binding are quantified on anti-HA immunoprecipitated DNA. (B) The same chromatin extracts were analyzed for Yox1 binding with anti-Yox1 polyclonal antibodies.