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. 2013 Nov 1;24(21):3381–3392. doi: 10.1091/mbc.E13-06-0330

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© 2013 Garbett et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — The tail of EBP50 contains a unique dynamic property that is absent in E3KARP. (A) Sequence alignment of human EBP50 and E3KARP. Conserved residues are highlighted in blue; the site where the tails of EBP50 and E3KARP were swapped is marked in red. (B) Photobleaching recovery curves of GFP-tagged EBP50, EBP50-E3tail, E3KARP, and E3KARP-50tail. Error bars show SD; n ≥ 9 for all experiments. (C) Representative time points from photobleaching experiments of GFP-tagged EBP50, E3KARP, EBP50-E3tail, and E3KARP-50tail. Photobleached areas are indicated by green boxes. Scale bars: 2 μm. (D) 3xFLAG-tagged EBP50 and E3KARP constructs expressed in JEG-3 cells were immunoprecipitated (IP) and blotted for FLAG, endogenous ezrin, and E-cadherin as a control.