TABLE 1:
Effects of drug inhibition on dHL-60 cell and primary neutrophil chemotaxis.
Cells | Parameter | DMSO | Rapamycin | Torin1 |
---|---|---|---|---|
dHL-60 | Speed (μm/min) | 4.36 ± 0.16 | 4.39 ± 0.12 | 4.67 ± 0.13 |
Chemotactic index | 0.97 ± 0.01 | 0.95 ± 0.01 | 0.96 ± 0.01 | |
Primary neutrophil | Speed (μm/min) | 7.05 ± 0.12 | 7.40 ± 0.55 | 7.47 ± 0.21 |
Chemotactic index | 0.96 ± 0.01 | 0.97 ± 0.01 | 0.96 ± 0.01 |
Cells were pretreated with DMSO, Torin1 (250 nM, 30 min), and rapamycin (100 nM, 30 min) before chemotaxis assay. The chemotactic index is defined as the ratio of migration in the correct direction to the actual length of the migration path (Xu et al., 2005).