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. Author manuscript; available in PMC: 2013 Oct 31.
Published in final edited form as: Nano Lett. 2008 Aug 29;8(10):10.1021/nl8012665. doi: 10.1021/nl8012665

Figure 4.

Figure 4

FACSgating of Oct4 GFP+ undifferentiated hESCs and RFP+ transfected hESCs for (a) free DNA and (b) C32-122/DNA nanoparticles. (c) Transfection of hESC colonies for different poly(beta-amino esters) (mean + SD). C32-117 (p<0.01) and C32-118 (p<0.05) are significantly better at gene delivery than Lipofectamine 2000. Micrograph of representative hESC colony, one week post-transfection with C32-122/DNA nanoparticles by (d) light microscopy, (e) GFP fluorescence (showing undifferentiation) and (f) RFP fluorescence (showing persistence of transfection).