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RNAi constructs. Fragments of the genes to be targeted by RNAi were cloned in diverging orientation in pKannibal. The RNAi constructs were then transferred into pPF408 so as to be under the control of the pDMC1 promoter. pPF408 also contains the T-DNA left and right borders (LB and RB), and the BastaR cassette to allow transformation and selection of transformed plants.
