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IL-1b (100 pg/ml) increased the immunostaining of CRM1 in astrocytes after 24 hrs. HMGB1 staining also showed evidence of translocation from nucleus into cytoplasm. Both CRM1 upregulation and HMGB1 translocation was inhibited by U0126 (10 μM). Cells were stained for the anti-CRM1 antibody (green), anti-HMGB1 antibody (red), and a nuclear stain DAPI (blue). Scale bar indicates 20 μm.
