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. 2004 Mar 11;23(6):1289–1300. doi: 10.1038/sj.emboj.7600156

Figure 7.

Figure 7

Mid1 stabilises spindle alignment to the longitudinal axis of the cell. (A) mid1Δ gfp-atb2 cells were filmed in medium containing Hoechst. Numbers indicate the time from when the spindle attained 2 μm. D1–D4 are images of chromatin staining. (B) Analysis of the changes in spindle angle (open circles) and spindle length (closed squares) from (A). The vertical bar denotes the time of chromosome separation (anaphase A). (C) Synchronous mid1-gfp cdc11-cfp cells placed in fresh medium containing Hoechst either in the absence (left panels) or presence (right panels) of 10 μm latrunculin B. Images were taken to visualise Cdc11-CFP (red), Mid1-GFP (green) or chromatin (blue) when cells were in G2 (single SPB), metaphase (separated SPBs but unseparated chromatin), anaphase (separated SPBs and separated chromatin) and telophase.