Table 1.
Frequency of misoriented spindles (%) | Length of phase 2 (min) | Rate of spindle elongation (μm min−1) | ||
---|---|---|---|---|
Before anaphase | After anaphase | |||
wt | 30 | 5 | 9±1 | 0.51±0.04 |
wt+Lat B | 52 | 27 | 18±4 | 0.30±0.02 |
cps8-188 | 92 | 28 | 24±4 | 0.31±0.02 |
myo52Δ | 75 | 30 | 52±23a | 0.26±0.05 |
myo51Δ | 87 | 40 | 24±2 | 0.28±0.03 |
cdc11-123 | 96 | 26 | 14±2 | 0.37±0.01 |
Percentage of misoriented spindles before and after chromosome separation in control cells (wt) or cells treated with latrunculin B (wt+Lat B) and in cps8-188, myo51Δ, myo52Δ, cdc11-123 and mid1Δ mutants. n=200 in each case. Statistical analysis of the timing of anaphase onset and rate of spindle elongation in wild type (n=20), wild type+Lat B (n=9), cps8-188 (n=6), myo51Δ (n=7), myo52Δ (n=8) and cdc11-123 (n=9). The error denotes standard error from the mean (SEM). | ||||
aExaggerated anaphase delay is partially due to altered cell polarity. |