Figure 3.

Suppression of catecholamine release by plasmin-generated CgA fragments. PC12 cells or primary bovine adrenal cells were stimulated with 60 μM nicotine in the absence (solid bars) or presence of 1 μM human CgA, which was either untreated (light gray bars) or was incubated (at 37°C for 60 minutes) with 2 μM plasmin, followed by inactivation of the plasmin with 1000 U/ml Trasylol (dark gray bars). Net percentage of secretion is shown and is calculated as secretagogue-stimulated release minus basal release. Results are mean ± SEM; n = 6 for each experimental group. ^AP < 0.01, ^BP < 0.001 compared with nicotine alone.